Synthetic cannabinoid JWH-018 produced nausea-like behavior in rats via CB1 receptors with stress hormone activation

The synthetic cannabinoid JWH-018 (found in "Spice" products) produced conditioned gaping, a nausea indicator, in rats at doses of 1 and 3 mg/kg, an effect reversed by the CB1 antagonist rimonabant and accompanied by elevated stress hormones.

DeVuono, Marieka V et al.·Cannabis and cannabinoid research·2020·Preliminary EvidenceAnimal StudyAnimal Study
RTHC-02513Animal StudyPreliminary Evidence2020RETHINKTHC RESEARCH DATABASErethinkthc.com/research

Quick Facts

Study Type
Animal Study
Evidence
Preliminary Evidence
Sample
Not reported

What This Study Found

JWH-018 at 1 and 3 mg/kg produced conditioned gaping (nausea). The 3 mg/kg effect was reversed by rimonabant, confirming CB1 mediation. JWH-018 elevated serum corticosterone levels, indicating HPA axis activation. This parallels previous findings with high-dose THC.

Key Numbers

JWH-018 at 1 and 3 mg/kg induced conditioned gaping. Rimonabant reversed 3 mg/kg effect. Corticosterone elevated at 3 mg/kg vs. vehicle.

How They Did This

Rats received 3 daily conditioning trials pairing saccharin with JWH-018 (0, 0.1, 1, 3 mg/kg). Rimonabant pretreatment tested for CB1 involvement. Serum corticosterone analyzed after 3 daily JWH-018 injections.

Why This Research Matters

JWH-018 has been found in "Spice" products linked to cannabinoid hyperemesis syndrome. This study provides mechanistic evidence that synthetic cannabinoids produce nausea through CB1-mediated stress response activation.

The Bigger Picture

This supports the hypothesis that cannabinoid hyperemesis syndrome results from CB1-mediated HPA axis dysregulation, explaining why both high-dose THC and synthetic cannabinoids can produce paradoxical nausea.

What This Study Doesn't Tell Us

Animal model; conditioned gaping is a proxy for nausea; JWH-018 doses may not match human recreational exposure; only one synthetic cannabinoid tested.

Questions This Raises

  • ?Would other synthetic cannabinoids found in "Spice" produce similar nausea effects?
  • ?Could CRH antagonists prevent CHS symptoms in humans?

Trust & Context

Key Stat:
JWH-018 nausea was CB1-mediated and accompanied by elevated corticosterone
Evidence Grade:
Single animal study with mechanistic detail but limited to one synthetic compound.
Study Age:
Published in 2020.
Original Title:
Nausea-Induced Conditioned Gaping Reactions in Rats Produced by High-Dose Synthetic Cannabinoid, JWH-018.
Published In:
Cannabis and cannabinoid research, 5(4), 298-304 (2020)
Database ID:
RTHC-02513

Evidence Hierarchy

Meta-Analysis / Systematic Review
Randomized Controlled Trial
Cohort / Case-Control
Cross-Sectional / Observational
Case Report / Animal StudyOne case or non-human subjects
This study

Tests effects in animals (usually mice or rats), not humans.

What do these levels mean? →

Frequently Asked Questions

Why would a cannabinoid cause nausea when cannabis is used to treat nausea?

At low doses, cannabinoids reduce nausea. At high doses or with potent full agonists like JWH-018, they can paradoxically induce nausea through overstimulation of CB1 receptors and activation of the stress response. This is thought to underlie cannabinoid hyperemesis syndrome.

What is the connection to "Spice" products?

JWH-018 is one of the active ingredients found in synthetic cannabis products sold as "Spice." Unlike THC (a partial CB1 agonist), JWH-018 is a full agonist, making it more likely to produce severe effects including nausea and vomiting.

Read More on RethinkTHC

Cite This Study

RTHC-02513·https://rethinkthc.com/research/RTHC-02513

APA

DeVuono, Marieka V; Hrelja, Kelly M; Petrie, Gavin N; Limebeer, Cheryl L; Rock, Erin M; Hill, Matthew N; Parker, Linda A. (2020). Nausea-Induced Conditioned Gaping Reactions in Rats Produced by High-Dose Synthetic Cannabinoid, JWH-018.. Cannabis and cannabinoid research, 5(4), 298-304. https://doi.org/10.1089/can.2019.0103

MLA

DeVuono, Marieka V, et al. "Nausea-Induced Conditioned Gaping Reactions in Rats Produced by High-Dose Synthetic Cannabinoid, JWH-018.." Cannabis and cannabinoid research, 2020. https://doi.org/10.1089/can.2019.0103

RethinkTHC

RethinkTHC Research Database. "Nausea-Induced Conditioned Gaping Reactions in Rats Produced..." RTHC-02513. Retrieved from https://rethinkthc.com/research/devuono-2020-nauseainduced-conditioned-gaping-reactions

Access the Original Study

Study data sourced from PubMed, a service of the U.S. National Library of Medicine, National Institutes of Health.

This study breakdown was produced by the RethinkTHC research team. We analyze and report published research findings without making health recommendations. All interpretations are based solely on the published abstract and study data.