Genetic variations in dopamine genes interacted with early cannabis use to alter hippocampal brain structure

Early-onset cannabis users showed altered hippocampal volumes in specific subregions, and a dopamine transporter gene variant modified how cannabis affected overall hippocampal size.

Batalla, Albert et al.·Cannabis and cannabinoid research·2018·Preliminary EvidenceCross-Sectional
RTHC-01587Cross SectionalPreliminary Evidence2018RETHINKTHC RESEARCH DATABASErethinkthc.com/research

Quick Facts

Study Type
Cross-Sectional
Evidence
Preliminary Evidence
Sample
Not reported

What This Study Found

Researchers examined hippocampal brain structure in 59 young men aged 18-30, including 30 chronic cannabis users who started regular use before age 16 and 29 controls. They combined high-resolution brain imaging with genetic testing for three dopamine-related genes: COMT, DAT1, and BDNF.

Cannabis users showed emerging alterations in total hippocampal volume and in specific subregions (CA1, CA2/3, and CA4), with associations between the amount of cannabis use and the degree of volume changes.

The most novel finding was an interaction between cannabis use and the DAT1 dopamine transporter gene. Normally, different variants of DAT1 (associated with higher or lower dopamine availability) relate to hippocampal size in a predictable pattern. Cannabis exposure disrupted this normal gene-brain relationship, suggesting that cannabis interferes with how dopamine gene variants shape hippocampal development.

Key Numbers

59 young men (30 cannabis users, 29 controls). Ages 18-30. Cannabis users started regular use before age 16. Altered subregions: CA1, CA2/3, CA4. Cannabis x DAT1 interaction significant for total hippocampal volume and fissure subregion. DAT1 variants: 9/9R (high dopamine) and 10/10R (low dopamine).

How They Did This

This was a cross-sectional neuroimaging-genetics study of 59 male Caucasian young adults aged 18-30. Hippocampal volumes were manually traced and automatically segmented into subregions using high-resolution MRI. Participants were genotyped for COMT, DAT1, and BDNF polymorphisms. Cannabis users had begun regular use before age 16.

Why This Research Matters

The hippocampus is critical for memory and learning, and its development during adolescence makes it potentially vulnerable to cannabis exposure. This study shows that the effects of cannabis on hippocampal structure are not uniform but depend on an individual's genetic makeup, particularly genes controlling dopamine signaling. This could help explain why some young cannabis users develop cognitive problems while others do not.

The Bigger Picture

This study exemplifies the gene-environment interaction approach to understanding cannabis effects. Rather than asking whether cannabis damages the brain in general, it asks which brains are most affected and why. The dopamine system is particularly relevant because it mediates both the rewarding effects of cannabis and is critical for hippocampal function.

What This Study Doesn't Tell Us

The sample was small (59 participants) and restricted to male Caucasians, limiting generalizability. The cross-sectional design cannot determine whether hippocampal differences preceded or followed cannabis use. Cannabis use was measured retrospectively. Multiple genetic comparisons increase the risk of false-positive findings. Only three candidate genes were tested.

Questions This Raises

  • ?Could DAT1 genotyping identify adolescents at higher risk for cannabis-related hippocampal effects?
  • ?Do these structural changes correspond to measurable memory or learning deficits?
  • ?Are the hippocampal alterations reversible with cannabis cessation?

Trust & Context

Key Stat:
Cannabis disrupted the normal relationship between dopamine genes and hippocampal structure
Evidence Grade:
This is a small neuroimaging-genetics study providing preliminary evidence of gene-environment interactions affecting hippocampal structure.
Study Age:
Published in 2018. Neuroimaging-genetics approaches to cannabis research have continued to develop.
Original Title:
The Influence of DAT1, COMT, and BDNF Genetic Polymorphisms on Total and Subregional Hippocampal Volumes in Early Onset Heavy Cannabis Users.
Published In:
Cannabis and cannabinoid research, 3(1), 1-10 (2018)
Database ID:
RTHC-01587

Evidence Hierarchy

Meta-Analysis / Systematic Review
Randomized Controlled Trial
Cohort / Case-Control
Cross-Sectional / ObservationalSnapshot without intervening
This study
Case Report / Animal Study

A snapshot of a population at one point in time.

What do these levels mean? →

Frequently Asked Questions

Does cannabis affect the hippocampus?

This study found that early-onset cannabis users showed changes in specific hippocampal subregions (CA1, CA2/3, CA4) compared to non-users. The hippocampus is critical for memory and learning, and these alterations were associated with the amount of cannabis consumed.

Do genetics determine who is affected?

The study found that the DAT1 dopamine transporter gene modified how cannabis affected hippocampal size. This suggests that genetic variations in dopamine signaling may make some individuals more vulnerable to cannabis-related brain changes than others.

Read More on RethinkTHC

Cite This Study

RTHC-01587·https://rethinkthc.com/research/RTHC-01587

APA

Batalla, Albert; Lorenzetti, Valentina; Chye, Yann; Yücel, Murat; Soriano-Mas, Carles; Bhattacharyya, Sagnik; Torrens, Marta; Crippa, José A S; Martín-Santos, Rocío. (2018). The Influence of DAT1, COMT, and BDNF Genetic Polymorphisms on Total and Subregional Hippocampal Volumes in Early Onset Heavy Cannabis Users.. Cannabis and cannabinoid research, 3(1), 1-10. https://doi.org/10.1089/can.2017.0021

MLA

Batalla, Albert, et al. "The Influence of DAT1, COMT, and BDNF Genetic Polymorphisms on Total and Subregional Hippocampal Volumes in Early Onset Heavy Cannabis Users.." Cannabis and cannabinoid research, 2018. https://doi.org/10.1089/can.2017.0021

RethinkTHC

RethinkTHC Research Database. "The Influence of DAT1, COMT, and BDNF Genetic Polymorphisms ..." RTHC-01587. Retrieved from https://rethinkthc.com/research/batalla-2018-the-influence-of-dat1

Access the Original Study

Study data sourced from PubMed, a service of the U.S. National Library of Medicine, National Institutes of Health.

This study breakdown was produced by the RethinkTHC research team. We analyze and report published research findings without making health recommendations. All interpretations are based solely on the published abstract and study data.