Topical CBD Gel Reduced Pain During Sex for Women With Vestibulodynia

A topical gel combining CBD and the terpene myrcene significantly reduced dyspareunia in women with vestibulodynia compared to placebo over 60 days.

Murina, Filippo et al.·Biomedicines·2025·Preliminary EvidenceRandomized Controlled Trial
RTHC-07212Randomized Controlled TrialPreliminary Evidence2025RETHINKTHC RESEARCH DATABASErethinkthc.com/research

Quick Facts

Study Type
Randomized Controlled Trial
Evidence
Preliminary Evidence
Sample
N=40

What This Study Found

Women applying 5% CBD plus myrcene gel to the vulvar vestibule for 60 days experienced significantly greater reductions in pain during intercourse compared to placebo, though both groups showed some improvement in overall pain scores.

Key Numbers

40 women enrolled (20 active, 20 placebo); 60-day treatment period; significant improvement in dyspareunia VAS scores in the active group versus placebo.

How They Did This

Randomized double-blind placebo-controlled trial with 40 women (20 per group) applying active gel or placebo to the vulvar vestibule for 60 days, measuring dyspareunia, pain, and vestibular cotton swab test scores on a 0-10 VAS scale.

Why This Research Matters

Vestibulodynia affects a significant number of women and current treatments are limited. This is one of the first RCTs testing topical CBD specifically for vulvar pain, offering a potential non-hormonal, non-opioid option.

The Bigger Picture

Topical CBD is being explored for various pain conditions, and this trial adds to the small but growing body of RCT evidence for localized CBD applications. The inclusion of myrcene, a cannabis-derived terpene, reflects growing interest in entourage effects.

What This Study Doesn't Tell Us

Very small sample size (n=40) limits generalizability. The combined formulation makes it impossible to separate CBD effects from myrcene effects. Short follow-up period with no assessment of durability after stopping treatment.

Questions This Raises

  • ?Would CBD alone without myrcene produce the same results?
  • ?What is the optimal concentration and duration of treatment?
  • ?Would results hold in a larger, multi-site trial?

Trust & Context

Key Stat:
40 women, 60 days: CBD + myrcene gel beat placebo for dyspareunia relief
Evidence Grade:
Preliminary: Although this is an RCT (the gold standard design), the very small sample size of 40 participants limits the strength of the conclusions.
Study Age:
Published in 2025, reflecting current research.
Original Title:
Efficacy and Safety of Topical 5% Cannabidiol Plus Myrcene for the Treatment of Vestibulodynia: A Multi-Centric Randomized Controlled Trial.
Published In:
Biomedicines, 13(10) (2025)
Database ID:
RTHC-07212

Evidence Hierarchy

Meta-Analysis / Systematic Review
Randomized Controlled TrialGold standard for testing treatments
This study
Cohort / Case-Control
Cross-Sectional / Observational
Case Report / Animal Study

Participants are randomly assigned to treatment or placebo groups to test cause and effect.

What do these levels mean? →

Frequently Asked Questions

What is vestibulodynia?

Vestibulodynia is the most common form of vulvodynia, causing burning, irritation, and pain during intercourse localized to the vulvar vestibule. It involves neuropathic pain sensitization.

What role does myrcene play?

Myrcene is a terpene found in cannabis that may inhibit peripheral pain sensitization. In this study it was combined with CBD, making it impossible to isolate the individual contribution of each compound.

Read More on RethinkTHC

Cite This Study

RTHC-07212·https://rethinkthc.com/research/RTHC-07212

APA

Murina, Filippo; Ettore, Giuseppe; Fochesato, Cecilia; Castiglione, Maria Grazia; Caruso, Melania; Fonti, Ilenia; Savasi, Valeria. (2025). Efficacy and Safety of Topical 5% Cannabidiol Plus Myrcene for the Treatment of Vestibulodynia: A Multi-Centric Randomized Controlled Trial.. Biomedicines, 13(10). https://doi.org/10.3390/biomedicines13102440

MLA

Murina, Filippo, et al. "Efficacy and Safety of Topical 5% Cannabidiol Plus Myrcene for the Treatment of Vestibulodynia: A Multi-Centric Randomized Controlled Trial.." Biomedicines, 2025. https://doi.org/10.3390/biomedicines13102440

RethinkTHC

RethinkTHC Research Database. "Efficacy and Safety of Topical 5% Cannabidiol Plus Myrcene f..." RTHC-07212. Retrieved from https://rethinkthc.com/research/murina-2025-efficacy-and-safety-of

Access the Original Study

Study data sourced from PubMed, a service of the U.S. National Library of Medicine, National Institutes of Health.

This study breakdown was produced by the RethinkTHC research team. We analyze and report published research findings without making health recommendations. All interpretations are based solely on the published abstract and study data.