Suk-SaiYasna, a traditional Thai herbal formula containing cannabis leaves, reversed stress-induced cognitive impairment in mice by activating antioxidant pathways and reducing brain oxidative stress.
Researchers interested in traditional medicine validation and the neuroprotective potential of cannabis-containing herbal formulations.
Cognitive impairment reversed across three behavioral tests
What the researchers found
SSY treatment significantly improved learning and memory in chronically stressed mice across three behavioral tests (Y-maze, novel object recognition, Morris water maze). SSY reduced lipid peroxidation in the hippocampus and frontal cortex and restored SOD and CAT antioxidant enzyme activity. The mechanism involved the Nrf2/Keap1 signaling pathway, with THC and THCA identified as bioactive compounds alongside other herbal antioxidants.
Why it matters
This bridges traditional Thai medicine with modern neuropharmacology, showing that a centuries-old cannabis-containing formula has measurable neuroprotective effects through well-characterized molecular pathways. The multi-compound formula may have advantages over isolated cannabinoids.
The numbers in context
SSY improved performance on Y-maze, novel object recognition, and Morris water maze tests. Reduced lipid peroxidation in hippocampus and frontal cortex. Restored SOD and CAT activity. Upregulated HO-1 and NQO1 mRNA via Nrf2-Keap1 pathway. Active compounds: THC, THCA, gallic acid, myricetin, myristicin, piperine, costunolide, gingerol.
How the study worked
Mice were exposed to unpredictable chronic mild stress (UCMS) to induce cognitive impairment. SSY-treated groups received the herbal formula during the stress period. Behavioral tests assessed learning and memory. Brain tissue was analyzed for oxidative stress markers and antioxidant enzyme activity. Gene expression analysis examined the Nrf2/Keap1 pathway. Phytochemical analysis identified active compounds.
What this study cannot tell us
Mouse model of chronic stress does not perfectly replicate human cognitive decline. The formula contains many active compounds, making it difficult to attribute effects to cannabis specifically. SSY is a traditional preparation that may vary in composition. Dose translation to humans is uncertain.
How to read the evidence
Well-designed animal study with multiple behavioral and molecular endpoints. Preliminary evidence for the specific formula, though the multi-compound nature complicates interpretation.
When this study was published
Published in 2025.
The bigger picture
Traditional medicine systems worldwide have used cannabis in multi-herb formulations for centuries. Modern pharmacological validation of these traditional preparations could lead to standardized herbal medicines with evidence-based dosing and quality control.
Questions still open
- How much of SSY's cognitive protection comes from cannabis versus other ingredients? Could a standardized version of this formula be developed for human clinical trials?
Common questions
What is Suk-SaiYasna?
Is the cannabis or the other herbs doing the work?
Read the original research
Suk-SaiYasna Remedy, a Traditional Thai Medicine, Mitigates Stress-Induced Cognitive Impairment via Keap1-Nrf2 Pathway.
International journal of molecular sciences, 26(11)
Citation
Masraksa, Wuttipong; Daodee, Supawadee; Monthakantirat, Orawan; Boonyarat, Chantana; Khamphukdee, Charinya; Kwankhao, Pakakrong; Mading, Abdulwaris; Muenhong, Poowanarth; Maneenet, Juthamart; Awale, Suresh; Matsumoto, Kinzo; Chulikhit, Yaowared. (2025). Suk-SaiYasna Remedy, a Traditional Thai Medicine, Mitigates Stress-Induced Cognitive Impairment via Keap1-Nrf2 Pathway.. International journal of molecular sciences, 26(11). https://doi.org/10.3390/ijms26115388
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