Daily CBD Supplement Use Can Trigger Positive Drug Tests for Athletes

Ten weeks of daily broad-spectrum CBD supplementation produced detectable levels of WADA-prohibited cannabinoids in urine, with concentrations increasing after exercise.

Gillham, Scott H et al.·Medicine and science in sports and exercise·2026·Moderate Evidenceclinical-trial
RTHC-08278Clinical TrialModerate Evidence2026RETHINKTHC RESEARCH DATABASErethinkthc.com/research

Quick Facts

Study Type
clinical-trial
Evidence
Moderate Evidence
Sample
N=36

What This Study Found

Daily use of a broad-spectrum CBD product (150 mg/day) led to detectable urinary concentrations of CBG in 42% and CBDV in 68% of pre-exercise samples, rising to 74% and 84% post-exercise, despite no THC detection at any timepoint.

Key Numbers

150 mg/day CBD for 10 weeks; n=36; CBG detected in 42→74% of samples pre-to-post exercise; CBDV in 68→84%; significant post-exercise increases for CBD (p=0.043), CBG (p=0.0023), CBDV (p=0.033); zero THC detection

How They Did This

Randomized, placebo-controlled trial with 36 healthy individuals (31 CBD, 5 placebo) self-administering broad-spectrum CBD or placebo for 10 weeks, followed by a 90-minute moderate-intensity exercise bout with blood and urine sampling.

Why This Research Matters

Athletes using legal broad-spectrum CBD products may unknowingly test positive for prohibited cannabinoids under WADA rules, and exercise amplifies this risk — a critical finding for competitive sport.

The Bigger Picture

As CBD use grows among athletes for recovery and wellness, this study reveals an underappreciated anti-doping risk from trace cannabinoids in broad-spectrum products that regulators and sports organizations need to address.

What This Study Doesn't Tell Us

Small placebo group (n=5); single CBD product tested; exercise protocol was moderate intensity only; did not assess high-intensity or repeated exercise effects; short-term study without washout period assessment.

Questions This Raises

  • ?Would isolate CBD products avoid this risk entirely?
  • ?How long after stopping supplementation do prohibited cannabinoids remain detectable?
  • ?Does exercise intensity correlate with cannabinoid release?

Trust & Context

Key Stat:
Evidence Grade:
Randomized placebo-controlled design with objective biomarker outcomes, though limited by small sample size and single product tested.
Study Age:
Published 2026; reflects current CBD product formulations and WADA prohibited list.
Original Title:
Daily Use of a Broad-Spectrum Cannabidiol Supplement Produces Detectable Concentrations of Cannabinoids in Urine Prohibited by the World Anti-Doping Agency: An Effect Amplified by Exercise.
Published In:
Medicine and science in sports and exercise, 58(1), 121-131 (2026)
Database ID:
RTHC-08278

Evidence Hierarchy

Meta-Analysis / Systematic Review
Randomized Controlled Trial
Cohort / Case-Control
Cross-Sectional / ObservationalSnapshot without intervening
This study
Case Report / Animal Study
What do these levels mean? →

Frequently Asked Questions

Can CBD supplements cause a failed drug test in sports?

Yes — this study showed that broad-spectrum CBD products containing trace amounts of other cannabinoids like CBG and CBDV can produce detectable levels of WADA-prohibited substances in urine, even though THC was never detected.

Does exercise affect CBD drug test results?

Yes. Exercise significantly increased urinary concentrations of multiple cannabinoids including CBG and CBDV, likely due to release from fat tissue during physical activity.

Read More on RethinkTHC

Cite This Study

RTHC-08278·https://rethinkthc.com/research/RTHC-08278

APA

Gillham, Scott H; Cole, Paige L; Owens, Daniel J; Chester, Neil; Bampouras, Theodoros M; McCartney, Danielle; Gordon, Rebecca; McGregor, Iain S; Close, Graeme L. (2026). Daily Use of a Broad-Spectrum Cannabidiol Supplement Produces Detectable Concentrations of Cannabinoids in Urine Prohibited by the World Anti-Doping Agency: An Effect Amplified by Exercise.. Medicine and science in sports and exercise, 58(1), 121-131. https://doi.org/10.1249/MSS.0000000000003842

MLA

Gillham, Scott H, et al. "Daily Use of a Broad-Spectrum Cannabidiol Supplement Produces Detectable Concentrations of Cannabinoids in Urine Prohibited by the World Anti-Doping Agency: An Effect Amplified by Exercise.." Medicine and science in sports and exercise, 2026. https://doi.org/10.1249/MSS.0000000000003842

RethinkTHC

RethinkTHC Research Database. "Daily Use of a Broad-Spectrum Cannabidiol Supplement Produce..." RTHC-08278. Retrieved from https://rethinkthc.com/research/gillham-2026-daily-use-of-a

Access the Original Study

Study data sourced from PubMed, a service of the U.S. National Library of Medicine, National Institutes of Health.

This study breakdown was produced by the RethinkTHC research team. We analyze and report published research findings without making health recommendations. All interpretations are based solely on the published abstract and study data.