A non-psychoactive hemp seed extract slowed colorectal cancer cell growth by triggering metabolic stress

The oil polar extract (OPE) from the Codimono hemp cultivar induced metabolic stress in HT-29 colorectal cancer cells, decreasing ATP by approximately 40%, activating AMPK (a cellular energy sensor), and disrupting autophagic flux. This caused G1 phase cell cycle arrest without triggering apoptosis. Adding chloroquine (an autophagy inhibitor) enhanced antiproliferative effects by approximately 30%.

In vitro study characterizing the phenolic composition of a polar extract from cold-pressed hemp seed oil (Codimono cultivar, lacking THC). Effects on HT-29 colorectal cancer cells were evaluated for ATP levels, AMPK activation, autophagy markers, cell cycle distribution, and apoptosis. Combination with chloroquine was tested.

This study identifies a non-psychoactive hemp-derived extract that works through metabolic stress rather than direct cell killing. The synergy with autophagy inhibitors suggests a combination strategy that could be explored in more advanced cancer models.

The cancer research field increasingly focuses on metabolic vulnerabilities of tumor cells. This study connects hemp-derived compounds to metabolic stress pathways, offering a different angle from the more commonly studied THC and CBD effects on cancer.

In vitro only (cell lines, not tumors in animals or humans). Single cell line (HT-29). The specific bioactive compounds within the extract are not fully identified. No pharmacokinetic data on whether these compounds would reach tumors in a living organism.