Cannabinol (CBN) Shows Strongest Anti-Biofilm Activity Against MRSA Among Five Cannabinoids Tested

All five cannabinoids (CBD, THC, CBN, CBG, CBC) had MICs of 1-2 ug/mL against MRSA. CBN showed the most potent anti-biofilm activity, significantly reducing biofilm biomass and bacterial viability. CBN also induced the highest intracellular ROS levels. CBD was least effective in most anti-biofilm assays but caused the greatest membrane damage, suggesting different mechanisms of action among cannabinoids.

In vitro study testing five phytocannabinoids against MRSA biofilms using crystal violet staining, resazurin metabolic assay, ROS assay, and propidium iodide membrane integrity test.

MRSA is a major cause of hospital-acquired pneumonia with few treatment options. This comparative study identifies CBN as the most promising anti-biofilm cannabinoid and reveals that chemically similar cannabinoids work through different mechanisms.

The antibiotic resistance crisis demands novel approaches. Cannabinoids represent an untapped class of antimicrobials, and the finding that different cannabinoids attack bacteria through different mechanisms raises the possibility of synergistic combinations.

In vitro study only. Biofilm conditions may not replicate in vivo infections. Cannabinoid concentrations achievable at infection sites not established. No combination testing performed.