A specific cannabinoid-terpene combination reduced cancer-promoting damage from acid reflux in esophageal cells

The CBG:Phytol 1:5 ratio reduced DCA-induced DNA damage, preserved mitochondrial membrane potential, decreased reactive oxygen species, enhanced apoptosis in damaged cells, and reduced mutagenicity. Patient sample analysis showed CB1 receptor expression correlated with esophageal adenocarcinoma progression.

In vitro models using human esophageal epithelial cells exposed to deoxycholic acid (bile acid) and a Barrett's esophagus gastroesophageal reflux model. Various cannabinoid-terpene ratios tested. Patient-derived tissue samples analyzed for ECS receptor expression.

Esophageal adenocarcinoma arises from chronic acid reflux damage. If cannabinoid-terpene combinations can reduce the mutagenic damage that drives this progression, they could represent a preventive strategy for people with Barrett's esophagus, a known cancer precursor.

This study bridges the entourage effect concept with cancer prevention, showing that specific cannabinoid-terpene ratios are more effective than individual compounds. The CB1 receptor's correlation with cancer progression suggests the endocannabinoid system is already active in this disease process.

Preprint (not yet peer-reviewed). In vitro models do not replicate the complexity of chronic reflux disease. The CBG:Phytol ratio was found through screening, not mechanistic optimization. Patient tissue analysis was correlative.