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CBD Combined With Low-Dose THC Was Most Effective Against Migraine-Like Symptoms in Rats

Animal StudyPreliminary evidence
The takeaway

In female rat models of both acute and chronic migraine, a CBD/THC combination outperformed CBD alone, CBD/CBG, and triple combinations in reducing pain and light sensitivity.

People with migraine; headache researchers; medical cannabis formulators

CBD/THC suppressed chronic migraine-like pain through day 13 in rats

What the researchers found

CBD/THC combination produced the longest-lasting pain relief (up to 3 hours) and was the only compound that reduced light sensitivity in the CGRP-induced migraine model. In the chronic model, CBD/THC suppressed pain through day 13.

Why it matters

Migraine affects roughly 12% of the global population and current treatments are often inadequate.

The numbers in context

CBD alone: reduced pain at 30 min to 2 hours. CBD/THC: reduced pain up to 3 hours. In chronic model: CBD/THC suppressed pain through day 13.

How the study worked

Female Wistar rats received systemic CBD (30 mg/kg), CBD/CBG (2:1), CBD/0.3% THC, or CBD/CBG/THC. Acute and chronic migraine models used.

What this study cannot tell us

Animal model. Only female rats. Doses may not translate to human use.

How to read the evidence

Well-designed preclinical study with multiple models, but limited to female rats.

When this study was published

2025 study using current preclinical models

The bigger picture

This study provides controlled preclinical evidence that adding even a small amount of THC to CBD meaningfully improves migraine outcomes.

Questions still open

  • What is the optimal THC:CBD ratio for migraine treatment? Could a CBD/THC migraine treatment achieve regulatory approval?

Common questions

Why was CBD/THC better than CBD alone for migraine?
The small amount of THC (0.3%) may enhance CBD's actions through CB1 receptor engagement, which CBD alone does not strongly activate.
Can I use CBD/THC for my migraines based on this study?
This is a rat study and cannot be directly translated to human treatment. Clinical trials would be needed.

Read the original research

Efficacy of different cannabinoid compounds on migraine-like responses in female rats.

Cephalalgia : an international journal of headache, 45(10), 3331024251386794

Citation

da Luz, Fernanda Mariano Ribeiro; Kaup, Alexandre Ottoni; Baggio, Darciane Favero; Costa, Flavio Henrique de Rezende; Chichorro, Juliana Geremias. (2025). Efficacy of different cannabinoid compounds on migraine-like responses in female rats.. Cephalalgia : an international journal of headache, 45(10), 3331024251386794. https://doi.org/10.1177/03331024251386794

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