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Study breakdown

CBD shows no signs of abuse potential in mouse behavior tests

Animal StudyPreliminary evidence
The takeaway

In multiple behavioral tests in mice, CBD produced no rewarding effects, no withdrawal symptoms, no changes in movement, and no motivation to self-administer, suggesting it lacks drug abuse potential.

Regulators, CBD researchers, and consumers interested in CBD safety.

No reward, no withdrawal, no self-administration

What the researchers found

CBD (15, 30, and 60 mg/kg) failed to induce conditioned place preference (no rewarding effects). No withdrawal symptoms or motor changes appeared 12 hours after repeated dosing. CBD plasma was nearly undetectable at 12 hours. In an oral self-administration paradigm, mice showed equal motivation and consumption for CBD solution and plain water.

Why it matters

Concerns about CBD abuse potential have slowed research and clinical development. This comprehensive behavioral profile helps address those concerns at the preclinical level.

The numbers in context

CPP tested at 15, 30, 60 mg/kg. Withdrawal assessed after 30 mg/kg twice daily for 6 days. Plasma measured at 2, 4, 8, 12, and 24 hours. Self-administration: 50 mg/kg oral CBD vs water.

How the study worked

Multiple behavioral paradigms in C57BL/6J mice: conditioned place preference (CPP) at three doses, spontaneous withdrawal assessment after 6 days of treatment, locomotor activity testing, plasma concentration measurements, and an oral self-administration paradigm comparing CBD water to plain water.

What this study cannot tell us

Animal study (mice); human abuse potential may differ. Specific doses and routes may not reflect human use patterns. Oral self-administration is less sensitive than intravenous paradigms.

How to read the evidence

Well-designed preclinical study with multiple complementary behavioral paradigms, but animal data only.

When this study was published

2019 preclinical study.

The bigger picture

With CBD products widely available to consumers, establishing that CBD lacks abuse potential at the preclinical level supports its continued availability and further therapeutic development.

Questions still open

  • Would extremely high CBD doses produce any rewarding effects? Do these findings hold across different mouse strains and species?

Common questions

Is CBD addictive?
This mouse study found no evidence of abuse potential: CBD did not produce rewarding effects, withdrawal symptoms, or motivation to self-administer, across multiple testing methods.
Can you get high from CBD?
In these animal tests, CBD produced no conditioned place preference (a measure of rewarding/euphoric effects) and mice showed no more interest in consuming CBD than plain water.

Read the original research

Cannabidiol does not display drug abuse potential in mice behavior.

Acta pharmacologica Sinica, 40(3), 358-364

Citation

Viudez-Martínez, Adrián; García-Gutiérrez, María S; Medrano-Relinque, Juan; Navarrón, Carmen M; Navarrete, Francisco; Manzanares, Jorge. (2019). Cannabidiol does not display drug abuse potential in mice behavior.. Acta pharmacologica Sinica, 40(3), 358-364. https://doi.org/10.1038/s41401-018-0032-8

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