CBD shows no signs of abuse potential in mouse behavior tests

CBD (15, 30, and 60 mg/kg) failed to induce conditioned place preference (no rewarding effects). No withdrawal symptoms or motor changes appeared 12 hours after repeated dosing. CBD plasma was nearly undetectable at 12 hours. In an oral self-administration paradigm, mice showed equal motivation and consumption for CBD solution and plain water.

Multiple behavioral paradigms in C57BL/6J mice: conditioned place preference (CPP) at three doses, spontaneous withdrawal assessment after 6 days of treatment, locomotor activity testing, plasma concentration measurements, and an oral self-administration paradigm comparing CBD water to plain water.

Concerns about CBD abuse potential have slowed research and clinical development. This comprehensive behavioral profile helps address those concerns at the preclinical level.

With CBD products widely available to consumers, establishing that CBD lacks abuse potential at the preclinical level supports its continued availability and further therapeutic development.

Animal study (mice); human abuse potential may differ. Specific doses and routes may not reflect human use patterns. Oral self-administration is less sensitive than intravenous paradigms.