Hempseed and Hempseed Oil Were Well-Tolerated in Laying Hen Diets

Laying hens fed diets containing up to 30% hempseed or 9% hempseed oil showed no negative effects on performance, egg quality, or most blood markers.

Neijat, M et al.·Poultry science·2014·Moderate EvidenceRandomized Controlled Trial
RTHC-00840Randomized Controlled TrialModerate Evidence2014RETHINKTHC RESEARCH DATABASErethinkthc.com/research

Quick Facts

Study Type
Randomized Controlled Trial
Evidence
Moderate Evidence
Sample
Not reported

What This Study Found

Forty-eight laying hens fed hemp-containing diets for 12 weeks showed no significant differences from control-fed hens in feed intake, rate of lay, egg weight, body weight gain, or egg quality. Blood metabolites including proteins, glucose, uric acid, cholesterol, and electrolytes were unaffected.

Liver enzyme levels (gamma-glutamyl transferase and aspartate aminotransferase) were actually lowest in hens fed moderate levels of hempseed (10% and 20%) and low hempseed oil (4.5%), suggesting a possible protective effect on liver health at moderate inclusion levels compared to higher levels or the control diet.

All diets were formulated to contain similar levels of crude fat (11%), energy (2,800 kcal/kg), and crude protein (17%), ensuring that differences were attributable to the hemp products rather than macronutrient imbalances.

Key Numbers

48 hens, 6 diet groups, 12-week study. Hempseed: 10%, 20%, 30%. Hempseed oil: 4.5%, 9.0%. No effects on performance or egg quality. Lower liver enzymes at 10-20% hempseed and 4.5% hempseed oil.

How They Did This

Forty-eight Lohmann LSL-Classic white-egg laying hens (19 weeks old) were individually caged and fed one of six diets for 12 weeks: hempseed at 10%, 20%, or 30%; hempseed oil at 4.5% or 9.0%; or a corn oil-based control. Performance, egg quality, and blood biochemistry were measured using repeated measures analysis.

Why This Research Matters

Hemp products are a potential sustainable feed ingredient for poultry, offering omega fatty acids and protein. This study provides safety data supporting the use of hempseed and hempseed oil in laying hen diets without negative impacts on production or animal health.

The Bigger Picture

As hemp cultivation expands following regulatory changes, the animal feed industry is exploring hemp products as sustainable protein and oil sources. This study adds to a body of evidence supporting the safety of hemp in poultry diets, which is relevant to both agricultural sustainability and the regulatory approval process.

What This Study Doesn't Tell Us

The sample size was small (8 hens per diet group). The study lasted only 12 weeks, so longer-term effects were not assessed. The protective liver enzyme finding was observational and the mechanism was not investigated. Results from one breed of laying hen may not generalize to all poultry.

Questions This Raises

  • ?What mechanism explains the apparent liver-protective effect of moderate hempseed inclusion?
  • ?Do hemp-fed hen eggs have different fatty acid profiles that could benefit consumers?
  • ?Would longer-term feeding studies reveal any cumulative effects?

Trust & Context

Key Stat:
No negative effects on egg quality or hen performance at any hemp inclusion level
Evidence Grade:
This is a randomized controlled feeding trial, but with a small sample size (48 hens across 6 groups).
Study Age:
Published in 2014. Hemp in animal feed has gained increasing regulatory attention since.
Original Title:
Performance, egg quality, and blood plasma chemistry of laying hens fed hempseed and hempseed oil.
Published In:
Poultry science, 93(11), 2827-40 (2014)
Database ID:
RTHC-00840

Evidence Hierarchy

Meta-Analysis / Systematic Review
Randomized Controlled TrialGold standard for testing treatments
This study
Cohort / Case-Control
Cross-Sectional / Observational
Case Report / Animal Study

Participants are randomly assigned to treatment or placebo groups to test cause and effect.

What do these levels mean? →

Frequently Asked Questions

Does hempseed contain THC?

Hempseed itself contains negligible THC. Industrial hemp varieties are bred to contain less than 0.3% THC in the plant, and the seed has even lower levels. This study focused on nutritional hemp products, not cannabinoid-containing extracts.

Could hemp-fed eggs contain cannabinoids?

This study did not measure cannabinoid levels in eggs. Given the negligible THC content in hempseed, transfer to eggs would be expected to be minimal, but direct measurement would be needed to confirm this.

Read More on RethinkTHC

Cite This Study

RTHC-00840·https://rethinkthc.com/research/RTHC-00840

APA

Neijat, M; Gakhar, N; Neufeld, J; House, J D. (2014). Performance, egg quality, and blood plasma chemistry of laying hens fed hempseed and hempseed oil.. Poultry science, 93(11), 2827-40. https://doi.org/10.3382/ps.2014-03936

MLA

Neijat, M, et al. "Performance, egg quality, and blood plasma chemistry of laying hens fed hempseed and hempseed oil.." Poultry science, 2014. https://doi.org/10.3382/ps.2014-03936

RethinkTHC

RethinkTHC Research Database. "Performance, egg quality, and blood plasma chemistry of layi..." RTHC-00840. Retrieved from https://rethinkthc.com/research/neijat-2014-performance-egg-quality-and

Access the Original Study

Study data sourced from PubMed, a service of the U.S. National Library of Medicine, National Institutes of Health.

This study breakdown was produced by the RethinkTHC research team. We analyze and report published research findings without making health recommendations. All interpretations are based solely on the published abstract and study data.