CBD Reduced Fear-Related Urination in Cats Exposed to Simulated Thunderstorms

Cats given CBD for two weeks showed significantly less fear-induced urination during thunderstorm simulations compared to placebo, suggesting CBD may help treat noise-induced fear in animals.

Masataka, Nobuo·Animals : an open access journal from MDPI·2025·Preliminary EvidenceRandomized Controlled Trial
RTHC-07072Randomized Controlled TrialPreliminary Evidence2025RETHINKTHC RESEARCH DATABASErethinkthc.com/research

Quick Facts

Study Type
Randomized Controlled Trial
Evidence
Preliminary Evidence
Sample
N=40

What This Study Found

After two weeks of CBD administration (4.0 mg/kg/day), cats exposed to a simulated thunderstorm showed significantly reduced fear-related urination compared to their initial test. No such improvement was seen in the placebo group receiving sunflower oil.

Key Numbers

N = 40 cats (20 per group). CBD dose: 4.0 mg/kg/day for 14 days. Significant reduction in fear-related urination in CBD group only. Placebo group showed no change.

How They Did This

Randomized placebo-controlled study with 40 cats naive to the testing protocol. Cats were assigned to CBD (4.0 mg/kg/day for 2 weeks) or placebo (sunflower oil). Each group was exposed to a thunderstorm simulation test before and after the 2-week administration period. Fear-related urination was the primary behavioral outcome.

Why This Research Matters

Noise phobia is one of the most common behavioral problems in companion animals. This small but controlled study adds to the growing evidence that CBD may have anxiolytic effects across species, using a practical and clinically relevant fear stimulus.

The Bigger Picture

While this is a veterinary study, it contributes to the broader evidence base for CBD as an anxiolytic. Animal studies with naturally occurring fear behaviors (rather than artificially induced anxiety) may better reflect the real-world potential of CBD for anxiety management.

What This Study Doesn't Tell Us

Small sample size. Only one behavioral measure (urination) was reported as significant. The study did not assess other fear behaviors like hiding, freezing, or vocalization in detail. Two weeks of administration makes it unclear whether shorter or longer treatment would differ.

Questions This Raises

  • ?Would CBD help with other types of animal anxiety beyond noise phobia?
  • ?Does the effect persist after CBD administration stops?

Trust & Context

Key Stat:
Significant reduction in fear-related behavior after 2 weeks of CBD
Evidence Grade:
Small randomized controlled trial with a relevant fear stimulus. Preliminary evidence limited by sample size and single behavioral measure.
Study Age:
Published in 2025.
Original Title:
Possible Anxiolytic Effects of Cannabidiol (CBD) Administration on Feline Responses to a Fear Response Test.
Published In:
Animals : an open access journal from MDPI, 15(11) (2025)
Database ID:
RTHC-07072

Evidence Hierarchy

Meta-Analysis / Systematic Review
Randomized Controlled TrialGold standard for testing treatments
This study
Cohort / Case-Control
Cross-Sectional / Observational
Case Report / Animal Study

Participants are randomly assigned to treatment or placebo groups to test cause and effect.

What do these levels mean? →

Frequently Asked Questions

Is CBD safe for cats?

This study used a specific dose (4.0 mg/kg/day) and did not report safety concerns. However, CBD metabolism differs between species, and pet owners should consult a veterinarian before administering CBD to animals.

Would this work for dogs with noise phobia?

The study was conducted in cats only. While CBD has shown anxiolytic effects in dogs in other studies, the specific dose and protocol would need to be validated separately for canines.

Read More on RethinkTHC

Cite This Study

RTHC-07072·https://rethinkthc.com/research/RTHC-07072

APA

Masataka, Nobuo. (2025). Possible Anxiolytic Effects of Cannabidiol (CBD) Administration on Feline Responses to a Fear Response Test.. Animals : an open access journal from MDPI, 15(11). https://doi.org/10.3390/ani15111642

MLA

Masataka, Nobuo. "Possible Anxiolytic Effects of Cannabidiol (CBD) Administration on Feline Responses to a Fear Response Test.." Animals : an open access journal from MDPI, 2025. https://doi.org/10.3390/ani15111642

RethinkTHC

RethinkTHC Research Database. "Possible Anxiolytic Effects of Cannabidiol (CBD) Administrat..." RTHC-07072. Retrieved from https://rethinkthc.com/research/masataka-2025-possible-anxiolytic-effects-of

Access the Original Study

Study data sourced from PubMed, a service of the U.S. National Library of Medicine, National Institutes of Health.

This study breakdown was produced by the RethinkTHC research team. We analyze and report published research findings without making health recommendations. All interpretations are based solely on the published abstract and study data.