Mice Showed Sleep Disruption After Stopping Cannabinoid Treatment

Mice that received a cannabinoid receptor agonist showed reduced total sleep time during light periods after the drug was stopped, providing an animal model for the sleep disturbances reported in human cannabis withdrawal.

Asano, Takashi et al.·Neuropsychopharmacology reports·2023·Preliminary EvidenceAnimal StudyAnimal Study
RTHC-04379Animal StudyPreliminary Evidence2023RETHINKTHC RESEARCH DATABASErethinkthc.com/research

Quick Facts

Study Type
Animal Study
Evidence
Preliminary Evidence
Sample
Not reported

What This Study Found

During cannabinoid administration, there was no difference in sleep/wake patterns between treated and control mice. However, after cessation, treated mice showed decreased total sleep time during the light period (when mice normally sleep), along with increased rearing behavior. This mirrors sleep disturbance symptoms reported in human cannabis withdrawal.

Key Numbers

EEG/EMG recorded for 3 days post-cessation; decreased total sleep during light period in ACPA mice; increased rearing behavior; no sleep differences during active ACPA administration

How They Did This

Mice were administered ACPA (a CB1 receptor agonist) or saline. EEG and EMG recordings were measured for 3 days after cessation. Behavioral changes (rearing, rubbing) were also assessed.

Why This Research Matters

Sleep disturbance is one of the most common and distressing symptoms of cannabis withdrawal, often driving relapse. Having an animal model to study this phenomenon enables testing of potential treatments.

The Bigger Picture

Cannabis withdrawal syndrome is now recognized as a clinical condition, but treatments are limited. Animal models like this one allow researchers to test medications that might alleviate withdrawal sleep disruption and potentially reduce relapse.

What This Study Doesn't Tell Us

Mouse sleep patterns differ from human sleep. ACPA is a synthetic agonist, not THC or whole cannabis. Short observation period of 3 days after cessation. Small sample size. Cannot directly translate timing or severity to human withdrawal.

Questions This Raises

  • ?How long do sleep disturbances persist after cessation?
  • ?Would sleep medications reduce other withdrawal symptoms?
  • ?Does the type of cannabinoid used affect withdrawal sleep patterns?

Trust & Context

Key Stat:
Sleep reduced after cessation only
Evidence Grade:
Controlled animal study with objective EEG/EMG measures, but limited translatability to human cannabis withdrawal
Study Age:
2023 study
Original Title:
Sleep disturbance after cessation of cannabis administration in mice.
Published In:
Neuropsychopharmacology reports, 43(4), 505-512 (2023)
Database ID:
RTHC-04379

Evidence Hierarchy

Meta-Analysis / Systematic Review
Randomized Controlled Trial
Cohort / Case-Control
Cross-Sectional / Observational
Case Report / Animal StudyOne case or non-human subjects
This study

Tests effects in animals (usually mice or rats), not humans.

What do these levels mean? →

Frequently Asked Questions

Does stopping cannabis cause sleep problems?

This mouse study supports what humans report: sleep disturbance after stopping cannabinoid use. Treated mice showed reduced sleep during their normal rest period after the drug was stopped.

Did the cannabinoid improve sleep while it was being taken?

No. There was no difference in sleep patterns between treated and control mice during active cannabinoid administration. The sleep disruption appeared only after the drug was stopped.

Read More on RethinkTHC

Cite This Study

RTHC-04379·https://rethinkthc.com/research/RTHC-04379

APA

Asano, Takashi; Takemoto, Hiroki; Horita, Tomoya; Tokutake, Tomohiro; Izuo, Naotaka; Mochizuki, Takatoshi; Nitta, Atsumi. (2023). Sleep disturbance after cessation of cannabis administration in mice.. Neuropsychopharmacology reports, 43(4), 505-512. https://doi.org/10.1002/npr2.12329

MLA

Asano, Takashi, et al. "Sleep disturbance after cessation of cannabis administration in mice.." Neuropsychopharmacology reports, 2023. https://doi.org/10.1002/npr2.12329

RethinkTHC

RethinkTHC Research Database. "Sleep disturbance after cessation of cannabis administration..." RTHC-04379. Retrieved from https://rethinkthc.com/research/asano-2023-sleep-disturbance-after-cessation

Access the Original Study

Study data sourced from PubMed, a service of the U.S. National Library of Medicine, National Institutes of Health.

This study breakdown was produced by the RethinkTHC research team. We analyze and report published research findings without making health recommendations. All interpretations are based solely on the published abstract and study data.