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Study breakdown

Cannabis Extract Reduced Cocaine Relapse in Mice

Animal StudyPreliminary evidence
The takeaway

A non-psychoactive cannabis extract sped up extinction of cocaine preference and blocked cocaine-primed relapse in mice, working through serotonin receptors rather than cannabinoid receptors.

Addiction researchers, scientists studying cannabinoid therapeutics, substance use treatment developers

Cannabis extract blocked cocaine relapse; pure CBD did not

What the researchers found

A non-psychoactive cannabis extract (NPCE) significantly reduced the extinction latency of smoked cocaine-induced place preference, while pure CBD did not. NPCE also selectively blocked relapse triggered by a priming dose of cocaine but not stress-induced relapse. The serotonin 5-HT1A receptor was involved in this effect, while the CB2 receptor was not.

Why it matters

Smoked cocaine (crack) is particularly addictive and difficult to treat. Finding that a non-psychoactive cannabis extract reduces relapse markers in a relevant animal model opens a potential new treatment avenue.

The numbers in context

NPCE reduced extinction latency of AEME-COC preference (CBD did not). NPCE blocked priming-dose reinstatement but not stress-induced reinstatement. 5-HT1A receptor antagonist attenuated NPCE effects. CB2 receptor inverse agonist had no significant impact.

How the study worked

Mouse conditioned place preference model using AEME-COC (a model for smoked cocaine). Three experiments tested: (1) cocaine vs AEME-COC conditioning, (2) CBD vs NPCE on extinction, (3) receptor antagonists on NPCE-mediated inhibition of reinstatement. Behavioral pharmacology approaches identified receptor mechanisms.

What this study cannot tell us

Animal model of cocaine preference does not fully capture human addiction. NPCE composition may vary between preparations. IP administration does not mimic human use. Single study requiring replication.

How to read the evidence

Preliminary: animal study using a conditioned place preference model with a single non-psychoactive cannabis extract preparation

When this study was published

Published in 2025

The bigger picture

The fact that a whole cannabis extract worked where isolated CBD did not suggests that other non-psychoactive compounds in the plant may contribute to anti-addiction effects. The serotonin mechanism provides a pathway for future drug development.

Questions still open

  • Which specific non-psychoactive compounds in the extract are responsible? Would oral NPCE administration produce the same effects? Could this approach translate to human crack cocaine treatment?

Common questions

Why did the cannabis extract work but pure CBD did not?
Cannabis contains many non-psychoactive compounds beyond CBD. The extract may contain a combination of cannabinoids, terpenes, and other molecules that work together to produce effects that CBD alone cannot achieve.
Could cannabis-based treatments help with cocaine addiction?
This animal study suggests a non-psychoactive cannabis extract may reduce some markers of cocaine addiction in mice. However, translating animal findings to human treatment requires extensive further research, including clinical trials.

Read the original research

Non-psychoactive cannabis extract promotes extinction and reduces reinstatement by priming dose in smoked cocaine-induced conditioned place preference.

Physiology & behavior, 301, 115048

Citation

Barreto, Fabián Leonardo; Lozano, María Constanza; Martínez-Ramírez, Jorge A. (2025). Non-psychoactive cannabis extract promotes extinction and reduces reinstatement by priming dose in smoked cocaine-induced conditioned place preference.. Physiology & behavior, 301, 115048. https://doi.org/10.1016/j.physbeh.2025.115048

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