rethinkTHC Search
Menu
Study breakdown

Cannabis reduced sperm motility and testosterone in diabetic rats

Animal StudyPreliminary evidence
The takeaway

In diabetic male rats, cannabis treatment caused a dose-dependent decrease in sperm motility at higher doses, and both cannabis and metformin negatively affected testosterone levels.

Diabetic men using cannabis, endocrinologists, and researchers studying cannabis effects on fertility.

Significant sperm motility decrease at 5 mg/kg THC dose

What the researchers found

Cannabis induced a significant concentration-dependent decrease in sperm motility at 5 mg/kg THC. Metformin increased sperm counts and lactate dehydrogenase activity. Both cannabis and metformin negatively affected testosterone concentrations.

Why it matters

Cannabis use is reportedly increasing among people with type 2 diabetes for pain and inflammation management. This study suggests cannabis may compound reproductive risks already associated with diabetes.

The numbers in context

35 rats. Cannabis 5 mg/kg THC: significant decrease in sperm motility (p=0.009). Metformin: increased sperm counts (p=0.035) and LDH activity (p=0.002). Both cannabis and metformin reduced testosterone.

How the study worked

Male Wistar rats (n=35) were fed a high-fat diet and injected with streptozotocin to create a type 2 diabetes model. Treatment groups received cannabis at THC concentrations of 1.25, 2.5, and 5 mg/kg and metformin (50 mg/kg) every other day for 10 weeks. Measured organ weight, testosterone, sperm count, motility, and enzyme activities.

What this study cannot tell us

Animal study in a chemically induced diabetes model. THC doses and frequency may not match human use patterns. Small sample (n=35 across all groups). Short duration relative to human chronic use.

How to read the evidence

Preliminary: small animal study with chemically induced diabetes model.

When this study was published

Published in 2022.

The bigger picture

Diabetic men already face reproductive challenges. If cannabis worsens sperm motility and testosterone in this population, it adds an important consideration for the growing number of diabetic patients turning to cannabis.

Questions still open

  • Are these effects reversible after stopping cannabis? Would lower doses be safe for reproductive parameters? Do diabetic men who use cannabis show similar fertility effects?

Common questions

Did all cannabis doses affect sperm?
Only the highest dose (5 mg/kg THC) significantly reduced sperm motility. Lower doses (1.25 and 2.5 mg/kg) did not show significant effects.
Did cannabis affect testosterone?
Yes. Both cannabis and metformin negatively affected testosterone concentrations, which is concerning because diabetes itself can already reduce testosterone levels.
Should diabetic men avoid cannabis?
This animal study raises caution but cannot make clinical recommendations. The researchers advise that cannabis should be used cautiously as an alternative treatment in diabetic males pending further research.

Read the original research

Cannabis and metformin on diabetic male Wistar rat sperm and reproductive organ parameters.

Journal of diabetes and metabolic disorders, 21(2), 1451-1458

Citation

van Losenoord, Wynand; Levendal, Ruby-Ann; Frost, Carminita Lara. (2022). Cannabis and metformin on diabetic male Wistar rat sperm and reproductive organ parameters.. Journal of diabetes and metabolic disorders, 21(2), 1451-1458. https://doi.org/10.1007/s40200-022-01079-z

Explore the wider topic