Long-Term Cannabis Use Leaves Marks on DNA, Dunedin Study Finds

The decades-long Dunedin birth cohort study found that long-term cannabis users had distinct DNA methylation patterns compared to non-users.

Meier, Madeline H et al.·Molecular psychiatry·2025·Strong EvidenceLongitudinal Cohort
RTHC-07111Longitudinal CohortStrong Evidence2025RETHINKTHC RESEARCH DATABASErethinkthc.com/research

Quick Facts

Study Type
Longitudinal Cohort
Evidence
Strong Evidence
Sample
Not reported

What This Study Found

Analysis of the Dunedin Multidisciplinary Health and Development Study revealed that decades of cannabis use was associated with specific DNA methylation changes, a type of epigenetic modification that can alter gene expression without changing the DNA sequence itself.

Key Numbers

Drawn from the Dunedin Study, a birth cohort followed for decades; specific methylation sites and effect sizes in full text.

How They Did This

Longitudinal birth cohort study (Dunedin Study) following participants from birth, with DNA methylation profiling conducted to compare long-term cannabis users with non-users.

Why This Research Matters

Epigenetic changes represent a biological mechanism through which cannabis exposure could have lasting effects on health, potentially affecting gene regulation in ways that persist beyond active use.

The Bigger Picture

Epigenetics is an emerging frontier in understanding how environmental exposures, including drug use, leave lasting biological imprints. These findings add cannabis to the list of substances with documented epigenetic signatures.

What This Study Doesn't Tell Us

Observational design cannot confirm cannabis directly caused the methylation changes. Other lifestyle factors correlated with cannabis use could contribute. Functional significance of identified methylation changes needs further study.

Questions This Raises

  • ?Do these methylation changes have clinical consequences?
  • ?Are they reversible after stopping cannabis use?
  • ?Could they be transmitted to offspring?

Trust & Context

Key Stat:
Decades-long Dunedin Study links cannabis use to DNA methylation changes
Evidence Grade:
One of the world's most rigorous longitudinal birth cohort studies, though observational design limits causal claims.
Study Age:
2025 analysis from the ongoing Dunedin Study, which began tracking participants at birth.
Original Title:
DNA methylation profiles of long-term cannabis users in midlife: a comprehensive evaluation of published cannabis-associated methylation markers in a representative cohort.
Published In:
Molecular psychiatry, 30(10), 4576-4590 (2025)
Database ID:
RTHC-07111

Evidence Hierarchy

Meta-Analysis / Systematic Review
Randomized Controlled Trial
Cohort / Case-ControlFollows or compares groups over time
This study
Cross-Sectional / Observational
Case Report / Animal Study

Follows a group of people over time to track how outcomes develop.

What do these levels mean? →

Frequently Asked Questions

Does cannabis change your DNA?

Not the DNA sequence itself, but this study found long-term cannabis use was associated with changes in DNA methylation, an epigenetic modification that affects how genes are expressed.

What is the Dunedin Study?

A world-renowned birth cohort study from New Zealand that has followed over 1,000 people from birth through middle age, providing some of the strongest longitudinal health data available.

Read More on RethinkTHC

Cite This Study

RTHC-07111·https://rethinkthc.com/research/RTHC-07111

APA

Meier, Madeline H; Sugden, Karen; Moffitt, Terrie E; Williams, Benjamin S; Bourassa, Kyle J; Houts, Renate; Ramrakha, Sandhya; Theodore, Reremoana; Caspi, Avshalom. (2025). DNA methylation profiles of long-term cannabis users in midlife: a comprehensive evaluation of published cannabis-associated methylation markers in a representative cohort.. Molecular psychiatry, 30(10), 4576-4590. https://doi.org/10.1038/s41380-025-03042-9

MLA

Meier, Madeline H, et al. "DNA methylation profiles of long-term cannabis users in midlife: a comprehensive evaluation of published cannabis-associated methylation markers in a representative cohort.." Molecular psychiatry, 2025. https://doi.org/10.1038/s41380-025-03042-9

RethinkTHC

RethinkTHC Research Database. "DNA methylation profiles of long-term cannabis users in midl..." RTHC-07111. Retrieved from https://rethinkthc.com/research/meier-2025-dna-methylation-profiles-of

Access the Original Study

Study data sourced from PubMed, a service of the U.S. National Library of Medicine, National Institutes of Health.

This study breakdown was produced by the RethinkTHC research team. We analyze and report published research findings without making health recommendations. All interpretations are based solely on the published abstract and study data.